diff --git a/tests/hwsim/test_eht.py b/tests/hwsim/test_eht.py
index 18ade87e3..1804c2250 100644
--- a/tests/hwsim/test_eht.py
+++ b/tests/hwsim/test_eht.py
@@ -1253,13 +1253,6 @@ def test_eht_5ghz_80mhz_2(dev, apdev):
     """EHT with 80 MHz channel width on 5 GHz - primary=149"""
     _test_eht_5ghz(dev, apdev, 149, 1, 155, 0)
 
-def test_eht_5ghz_80mhz_puncturing_override_1(dev, apdev):
-    """EHT with 80 MHz channel width on 5 GHz - primary=36 - puncturing override (2nd)"""
-
-    # The 2nd 20 MHz is punctured
-    _test_eht_5ghz(dev, apdev, 36, 1, 42, 0,
-                   eht_oper_puncturing_override="0x0002")
-
 def test_eht_5ghz_80mhz_puncturing_override_2(dev, apdev):
     """EHT with 80 MHz channel width on 5 GHz - primary=149 - puncturing override (3rd)"""