diff --git a/tests/hwsim/test_eht.py b/tests/hwsim/test_eht.py
index 1804c2250..18ade87e3 100644
--- a/tests/hwsim/test_eht.py
+++ b/tests/hwsim/test_eht.py
@@ -1253,6 +1253,13 @@ def test_eht_5ghz_80mhz_2(dev, apdev):
     """EHT with 80 MHz channel width on 5 GHz - primary=149"""
     _test_eht_5ghz(dev, apdev, 149, 1, 155, 0)
 
+def test_eht_5ghz_80mhz_puncturing_override_1(dev, apdev):
+    """EHT with 80 MHz channel width on 5 GHz - primary=36 - puncturing override (2nd)"""
+
+    # The 2nd 20 MHz is punctured
+    _test_eht_5ghz(dev, apdev, 36, 1, 42, 0,
+                   eht_oper_puncturing_override="0x0002")
+
 def test_eht_5ghz_80mhz_puncturing_override_2(dev, apdev):
     """EHT with 80 MHz channel width on 5 GHz - primary=149 - puncturing override (3rd)"""